Bio 152 lab
Lab one Blood
MATERIALS
Prepared slides of human blood with Wright’s or Giemsa stain
Compound microscopes
Lab charts or illustrations showing the various blood cell types
Latex gloves
Roll of paper towels
Vial of mammal blood or
Sterile cotton balls
Alcohol swabs
Sterile, disposable lancets
Adhesive bandages
Dropper bottle of Wright’s stain
Squeeze bottle of distilled water or phosphate buffer solution
Large finger bowl or staining tray
Toothpicks
Clean microscope slides
Coverslips
Pasteur pipette and bulbs
Hand counter
Biohazard bag or container
10% bleach container
Sharps container
Blood typing
MATERIALS
5 ml of fresh, non-human mammal blood (dog, sheep, or cow)
Sterilized human blood (Carolina #k3-70-0120 or other supply company), labeled 1 to 4
Blood typing antisera (anti-A, anti-B, anti-D), test cards, and toothpicks
Rh warming tray
Heparinized blood microcapillary tubes
Capillary tube centrifuge
Hematocrit reader (Criticorp Micro-hematocrit tube reader, Damon Micro-capillary reader, or mm ruler)
Seal-ease
300 mL Erlenmeyer flask
1000uL micropipette or 1 mL pipette with pipette pump
0.9% saline solution
Hemoglobinometer
Hemacytometer, coverglass
Microscope
Protective gloves
Goggles
Tallquist paper
Tallquist chart
Contamination bucket with autoclave bag
Container with 10% household bleach solution
Sharps container
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lab 2
The Cardiovascular System Structure of the Heart
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MATERIALS
Models and charts of the heart
Preserved sheep hearts
Preserved human hearts (if available)
Blunt probes (mall probes)
Dissection pans
Scalpels
Sharps container
Disposable gloves
Waste container
Microscopes
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Cardiovascular Sounds & Heart Rate Control
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MATERIALS
Pulse Rate and Heart Sounds
Clock or watch with second hand
Stethoscope
Alcohol wipes
Frog Heart Rate and Contraction Strength
Frogs
Clean dissection instruments:
Scissors
Razor blades or scalpels
Dissection tray
Forceps
Dissection pins
Small heart hook (fish hook, copper wire, or Z wire)
Nylon Thread
Frog anchoring board
Ring stand
Goggles
Animal waste container
Electrocardiogram Monitoring Chart records & Computerized Hardware
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MATERIALS
Cot or covered lab table
Alcohol swabs
Electrode jelly, paste, or saline pads
BIOPAC Section
EL 503—general purpose disposable electrode pads
SS2L—lead cables
CBLSERA—CBL serial for MP30
MP30—data acquisition unit and power supply
(AC100A)
Compatible computer
ECG Machine Section
Electrocardiograph machine
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MATERIALS
Microscopes
Prepared slides of arteries and veins
Models of the blood vessels of the body
Charts and illustrations of the arterial system
Cadaver
Microscope slides of arteries and veins in cross section
Cats
Dissection equipment:
Dissection tray or pan
Scalpels
Pins
Blunt (Mall) probes
Gloves
Scissors
Forceps
Animal waste container
First aid kit
MATERIALS
Microscope
Prepared slide of arteriosclerosis
Models of the blood vessels of the body
Charts and illustrations of arterial system
Cadaver
Cats
Materials for cat dissection:
Dissection trays
Scalpel
Gloves
Blunt (Mall) probe
String and tags
Pins
Forceps and sharp scissors
First aid kit in lab or prep area
Sharps container
Animal waste disposal container
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The Venous System and Blood Vessels and Blood Pressure
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MATERIALS
Models of the blood vessels of the body
Charts and illustrations of venous system
Cadaver (if available)
Cats
Materials for cat dissection:
Dissection trays
Scalpel and two to three extra blades
Gloves (household latex gloves work well for repeated use)
Blunt (Mall) probe
String and tags
Pins
Forceps and sharp scissors
First aid kit in lab or prep area
Sharps container
Animal waste disposal container
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Functions of Vessels and The Lymphatic System
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IME 1 - 1.5 hours
MATERIALS
Live frog
Dissection scopes
Paper towels
Small squeeze bottle of water
Microscopes
Microscope slides of lymphatic vessels with valves, lymph nodes
Charts, diagrams, and models of blood vessels
Torso models
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lab 8..The Respiratory System The Respiratory Organs
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MATERIALS
Lung models or detailed torso model, including midsagittal section of head
Model of larynx
Microscopes
Prepared microscope slides of lung tissue
Prepared microscope slides of "smoker's lung"
Charts and illustrations of the respiratory system
Cats
Materials for cat dissection:
Dissection trays
Scalpel and two to three extra blades
Gloves (household latex gloves work well for repeated use)
Blunt (Mall) probe
Forceps and sharp scissors
First aid kit in lab or prep area
Sharps container
Animal waste disposal container
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lab 9
| 9 |
Respiratory Function, Breathing, and Respiration
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MATERIALS
Respiration Model
Bell jar respiration model
Pulmonary Volume Setup
Biopac air transducer, wet spirometer, or handheld spirometers
Disposable mouthpieces to fit respirometer or spirometers
Watch or clock with accuracy in seconds
Biohazard bag
AFT6—600 mL calibration syringe
AFT1—disposable bacteriological filter
AFT2 disposable mouthpiece
SS11LA airflow transducer
Breathing Sounds and Breathing Rate Setup
Stethoscope
Alcohol wipes
Acid-Base Setup (one setup per table)
Litmus solution (2 g litmus powder in 600 ml water)
NaOH solution (1 N) in dropper bottles
Straws
100 ml Erlenmeyer flasks
Tape or parafilm
Safety glasses
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lab 10 midterm
| 10 |
MIDTERM EXAM - PRACTICAL & WRITTEN
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lab 11
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| The Anatomy of the Digestive System |
38 |
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Physiology of the Digestive System
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MATERIALS
Models, charts, or illustrations of the digestive system
Hand mirror
Cats
Dissection trays
Pins
Scalpels
Gloves
Animal waste container
Skull, human teeth, or cast of teeth
Cadaver (if available)
Microscopes
Microscope slides:
Esophagus
Stomach
Small intestine
Large intestine
Liver
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Digestive Physiology part
MATERIALS
Microscopes
General Supplies
80 test tubes (15 ml each)
12 test tube holders
6 test tube racks
12 test tube brushes and soap
Warm water bath, set at 37° C with test tube racks and thermometer
Hot water bath or hot plates and 400 ml beakers for hot bath (100°C) and thermometer
6 permanent markers
18 pipettes of 5 ml each
6 pipette pumps
7 100 ml beakers to pour from stock bottle for distribution
1 250 ml bottle of tap water
6 small spatulas
100 ml 1% pancreatin solution, fresh
300 ml 1% alpha amylase solution, fresh
100 ml 0.1% alpha amylase solution, fresh
300 ml Benedict’s reagent (6 50 ml bottles)
Parafilm squares (10 per table)
Biohazard container or container with 10% bleach solution
Starch Digestion
6 dropper bottles of iodine solution
250 ml of 0.5% potato starch solution
6 boxes of microscope slides (1 per table)
6 boxes of coverslips (1 per table)
6 eyedroppers
Sugar Test
100 ml of 1% maltose solution
6 10 ml graduated cylinders
Cellulose Digestion
Cellulose - 2 cotton balls cut into small pieces
6 small dropper bottles of water
Lipid Digestion
200 mL litmus cream
6 dropper bottles of "acid solution" (lemon juice or vinegar)
6 dropper bottles of 1% sodium hydroxide solution (NaOH)
12 pair of goggles (during use of NaOH)
12 pair of latex gloves
Protein Digestion
25 ml of 1% BAPNA solution; BAPNA (benzoyl-DL-arginine-p-nitroanilide) is available from Sigma B4875 or Aldrich 85,711-4
Surface Area and Digestion
2 large potatoes per class
2 breadboards
2 number 4 cork borers
2 small rods (applicator sticks) to push through the borers
12 rulers (15 cm)
6 razor blades
2 mortars and pestles
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lab 12
MATERIALS
Models and charts of the urinary system
Models and illustrations of the kidney and nephron system
Microscopes
Microscope slides of kidney and bladder
Samples of renal calculi (if available)
Preserved specimens of sheep or other mammal kidney
Dissection trays an
d materials
Scalpels
Forceps
Blunt (Mall) probes
Latex gloves
Waste container
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lab 13
The Male and Female Reproductive Systems |
MATERIALS
Charts, models, and illustrations of the male reproductive system
Microscopes
Prepared slides of a cross section of testis and sperm smear
Cats
Materials for cat dissection:
Dissection trays
Scalpel and two to three extra blades
Gloves (household latex gloves work well for repeated use)
Blunt (Mall) probe
Forceps and sharp scissors
First aid kit in lab or prep area
Sharps container
Animal waste disposal container
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Material needed for Bio 251 lab
Dear Ms Johnson for the Bio 251 labs I need the following. Next week will be week two on tuesdayThanks
Lab one
Acid/Base
Five 10 mL test tubes
Test tube rack
10 mL graduated cylinder
Permanent marker
Distilled water in dropper bottle
0.1 M HCl in dropper bottle
0.1 M NaOH in dropper bottle
Baking soda (sodium bicarbonate)
Sodium chloride (table salt)
Wide-ranging pH paper (pH 1-14)
Parafilm®
Small metal spatula
Balance and weigh paper
Ionic and Covalent Molecules
18 gauge wire
Alligator clips
9 volt battery
6 volt flashlight bulb
Miniature screw lamp receptacle (Carolina #756481 or Sargent Welch #CP 33008-00)
Two 50 mL beakers
15% sucrose solution in dropper bottle
15% sodium chloride solution in dropper bottle
Hydrogen Bonds
Graduated cylinder
Two 50 mL beakers
Small bottle of distilled water
Small bottle of ethanol (70% or greater)
Hot plate (do not use open flame)
Measurement Section
Meter stick - 1 per pair of students
Small metric ruler (30 cm) - 1 per table
Organ Systems Section
Models of Human Torso
Charts of Human Torso
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lab 2
Cell Structure and Function - osmosis, diffusion, filtration
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MATERIALS
Cell Structure
Models or charts of animal cells
Electron micrographs of cells or textbook with electron micrographs
Prepared slides of whitefish blastula
Microscopes
Modeling clay (Plasticine)—two colors
Marbles
Brownian Motion
Dilute solution of India ink in dropper bottles
Microscopes
Microscope slides
Coverslips
Hot plate
Diffusion Demonstration
Potassium permanganate crystals
100 ml beaker (one per table)
Water
Small tweezers or small spatula
Diffusion
Agar plates (three per table)
0.01 M potassium permanganate solution in dropper bottles
0.01 M methylene blue solution in dropper bottles
Plastic drinking straws
Millimeter ruler
Fine probe, spatula, or small forceps
Warming tray or incubator
Osmosis Demonstration
Thistle tube
Dialysis tubing
Rubber band
Molasses or concentrated sucrose solution (20%)
1% starch solution
Ring stand and clamp
250 ml beaker
Distilled water
China marker
Osmosis Experiment
Four strips of 20 cm long dialysis tubing (one set of four per table)
Four 200 ml beakers
String or “W” clamp with wooden applicator stick
Scissors
Four colored solutions (2 L each) of 0%, 5%, 15%, and 30% sucrose
1 liter of 15% sucrose solution
Balances
Towels
Pipettes (10 ml)
Pipette pumps
Osmosis and Living Cells
Clean glass microscope slides
Coverslips
5 ml of mammal blood (contact local veterinarian’s office)
Distilled water in dropper bottle (one per table)
0.9% saline solution in dropper bottle (one per table)
5% sodium chloride solution in dropper bottles (one per table)
Latex or plastic gloves
Filtration
Filter paper
Funnel
Ring stand with ring clamp
10 ml graduated cylinder
500 ml beaker
Iodine solution in dropper bottles (one per table)
Filtration solution (500 ml of 1% starch, 1% charcoal, and 1% copper sulfate) consists of 5 g each of starch, charcoal, and copper sulfate in one bottle or flask
Stopwatch or clock with second hand
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lab 3
MATERIALS
Microscope
Colored pencils
Epithelial Tissue Slides
Simple squamous epithelium
Simple cuboidal epithelium
Simple columnar epithelium
Stratified squamous epithelium
Transitional epithelium
Pseudostratified columnar epithelium
Muscular Tissue Slides
Skeletal muscle
Cardiac muscle
Smooth muscle
All three muscle types
Nervous Tissue Slides
Spinal cord smear
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| LABORATORY SESSION IV |
Tissues - Slides: Connective tissue proper, Osseous tissue (bone) Vascular tissue (blood), Cartilage
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Connective Tissue Slides
Dense regular connective tissue
Dense irregular connective tissue
Elastic connective tissue
Reticular connective tissue
Loose (areolar) connective tissue
Adipose tissue
Hyaline cartilage
Fibrocartilage
Elastic cartilage
Ground bone
Cancellous bone
Bone marrow
Blood
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| LABORATORY SESSION V |
Integumentary System
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MATERIALS
Models and charts of the integumentary system
Microscopes
Prepared microscope slides of:
Thick skin (with Pacinian corpuscles)
Hair follicles
Pigmented and nonpigmented skin
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LABORATORY SESSION VI - UNIFORM MIDTERM EXAM
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| LABORATORY SESSION VII |
Introduction to the Skeletal System: Axial and Appendicular Skeleton
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MATERIALS
Chart of the skeletal system
Cut sections of bone showing compact and spongy bone
Articulated human skeleton
Articulated cat skeleton (if available)
Disarticulated human skeleton
Prepared slide of ground bone
Prepared slide of decalcified bone
Bone heated in oven at 350°F for three hours or more
Bone placed in vinegar for several weeks or 1 N nitric acid for a few days.
Microscopes
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| LABORATORY SESSION VIII |
| App |
MATERIALS
Articulated skeleton or plastic cast of articulated skeleton
Disarticulated skeleton or plastic casts of bones
Charts of the skeletal system
Wooden applicator sticks or plastic drinking straws cut on a bias for pointer tips
Foam pads of various sizes (to protect real bones from hard countertops)
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| LABORATORY SESSION IX |
Axial Skeleton B Vertebrae, Ribs, Sternum, Hyoid Bones
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MATERIALS
Articulated skeleton or plastic casts of a skeleton
Disarticulated skeleton or plastic casts of bones
Charts of the skeletal system
Wooden applicator sticks or plastic straws cut on a bias for pointer tips
Foam pads of various sizes (to protect bone from hard countertops)
A cardboard box or piece of wood about 1 foot square and 3 inches deep
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| LABORATORY SESSION X |
Axial Skeleton B Skull
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MATERIALS
Disarticulated skull, if available
Articulated skulls with the calvaria cut
Fetal skulls
Foam pads to cushion skulls from the desktop
Pipe cleaners, wooden applicator sticks, or plastic straws
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| LABORATORY SESSION XI |
Articulations
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MATERIALS
Mammal joint with intact synovial capsule
Dissection tray with scalpel or razor blades, blunt probe, and protective gloves
Waste container
Model or chart of joints including those of the shoulder, elbow, knee, ankle, hip, and jaw
Articulated skeleton
Fetal skulls
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| LABORATORY SESSION XII |
Muscles of the Shoulders and Upper Extremity
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MATERIALS
Human torso model and upper extremity model
Human muscle charts
Articulated skeleton
Cadaver (if available)
Cat
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| LABORATORY SESSION XIII |
Muscles of the Hip, Thigh, Leg, and Foot
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MATERIALS
Human torso model
Human leg models
Human muscle charts
Articulated skeleton
Cadaver (if available)
Cat
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| LABORATORY SESSION XIV |
Muscles of the Head and Neck
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MATERIALS
Human torso model and head and neck model
Human muscle charts
Articulated skeleton
Cadaver (if available)
Cat
Cat wetting solution
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EXERCISE 15
Muscles of the Torso
